Single-molecule localization microscopy comes of age: From concepts to biological impact- Now Complimentary on Demand

Single-molecule localization microscopy comes of age: From concepts to biological impact- Now Complimentary on Demand In case you missed our live, online educational seminar, "Single-molecule localization microscopy comes of age: From concepts to biological impact" we wanted to let you know that it is available in our complimentary on-demand archive. You can access this archive to watch the webinar at any time. For more information and access to the archive, go to: webinar.sciencemag.org About This Webinar Superresolution microscopy by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cells function at a molecular level. Single-molecule localization microscopy (SMLM) can provide single-molecule information about the distribution and even the absolute numbers of proteins in subcellular compartments. During this webinar, our speakers will introduce the basic requirements of SMLM and its potential use for quantitative molecular imaging. Obstacles and how to bypass them will be discussed, as well as the advantages of direct stochastic optical reconstruction microscopy (dSTORM) for quantitative imaging of proteins. Furthermore, the influence of different environmental and artificial variables on the survival of different cell lines will be demonstrated. The speakers will present data on different image capture technologies for SMLM, focusing on electron multiplying charge-coupled device (EMCCD) and scientific complementary metal-oxide semiconductor (sCMOS) cameras, discussing frame rates and the need for sensitive detectors to harvest the comparatively low light levels associated with single-molecule fluorescence emission. Information on how to optimize your camera setup for superresolution imaging will also be provided. During the webinar, the viewers will: > Learn the basics of SMLM > Gain a better understanding of the challenges of SMLM and how to overcome them > Hear about the optimal image capture technologies for SMLM >Have their questions answered live by the panel! Participants: Markus Sauer, Ph.D. University of Würzburg Würzburg, Germany Christian Soeller, Ph.D. University of Exeter Exeter, United Kingdom Orla Hanrahan, Ph.D. Andor Technology Belfast, Ireland Register at: webinar.sciencemag.org Questions? E-mail: webinar@aaas.org. Produced by the Science/AAAS Custom Publishing Office and sponsored by Andor Technology.